The new facilities support the production of proteins in quantities and qualities suitable for the highest demands, such as: detailed biophysical and biochemical studies, structural studies (crystallization and X-ray crystallography, NMR spectroscopy), antibody and antigen generation, and manufacture of protein chips.

Our services are cost-effective and can be tailored to support extensive, new research directions or complement on-going laboratory efforts. In this initial phase, we currently provide the following services:

• protein expression using E. coli and baculovirus-mediated insect cell systems

• design and optimization of protein purification schemes using advanced chromatography systems

• guidance in biophysical characterization, including electrophoresis, light scattering and fluorescence spectroscopy, to ascertain protein structural integrity, monodispersity and functionality

In addition, we are dedicated to training investigators, post-doctoral fellows, graduate students and technical staff in expert use of the techniques and equipment available in our facilities. Please peruse our website at http://med.unc.edu/csb/PEP/ or contact us directly for further information.

Mischa Machius, PhD, Director, Center for Structural Biology, Associate Professor, Dept. of Pharmacology, Member, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill
4079 Genetic Medicine, CB# 7365
120 Mason Farm Road, Chapel Hill, NC 27599-7365
machius@med.unc.edu
Office: 919-843-4485

Michael J Miley, PhD, Director, Macromolecular X-Ray Crystallography and Protein Expression & Purification Facilities, Assistant Professor, Dept. of Pharmacology, University of North Carolina at Chapel Hill
Medical School Building B, Room 124 CB# 7365
Chapel Hill, NC 27599-7365
mjmiley@email.unc.edu
Office: 919-843-7174
Cell: 919-451-3593